Resource type
Thesis type
(Thesis) M.A.Sc.
Date created
2011-06-24
Authors/Contributors
Author (aut): Chhina, Sumanpreet Kaur
Abstract
This thesis investigates the design, fabrication, and testing of an easy-to-use, disposable and portable microfluidic system for DNA amplification detection; this is suitable for point-of-care testing (POCT) applications. The microfluidic system utilizes biotin-labelled DNA to agglutinate streptavidin-coated microspheres. The microfluidic system is designed to retain aggregates of cross-linked microspheres as opposed to single microspheres, indicating the detection of biotin-labelled DNA. The microfluidic platform is composed of a filter design and inlet/outlet reservoirs, which was fabricated using microfabrication techniques. This research demonstrates that the microfluidic system is an improvement on the current DNA detection technique utilizing particle agglutination. Such a system may in turn form the basis of future hand-held, compact, point-of-care biosensors for disease screening and identification.
Document
Identifier
etd6683
Copyright statement
Copyright is held by the author.
Scholarly level
Supervisor or Senior Supervisor
Thesis advisor (ths): Parameswaran, Ash
Member of collection
Download file | Size |
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etd6683_SChhina.pdf | 2.75 MB |