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Microfluidic system to detect select DNA fragments using agglutination process

Resource type
Thesis type
(Thesis) M.A.Sc.
Date created
2011-06-24
Authors/Contributors
Abstract
This thesis investigates the design, fabrication, and testing of an easy-to-use, disposable and portable microfluidic system for DNA amplification detection; this is suitable for point-of-care testing (POCT) applications. The microfluidic system utilizes biotin-labelled DNA to agglutinate streptavidin-coated microspheres. The microfluidic system is designed to retain aggregates of cross-linked microspheres as opposed to single microspheres, indicating the detection of biotin-labelled DNA. The microfluidic platform is composed of a filter design and inlet/outlet reservoirs, which was fabricated using microfabrication techniques. This research demonstrates that the microfluidic system is an improvement on the current DNA detection technique utilizing particle agglutination. Such a system may in turn form the basis of future hand-held, compact, point-of-care biosensors for disease screening and identification.
Document
Identifier
etd6683
Copyright statement
Copyright is held by the author.
Permissions
The author granted permission for the file to be printed and for the text to be copied and pasted.
Scholarly level
Supervisor or Senior Supervisor
Thesis advisor (ths): Parameswaran, Ash
Member of collection
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etd6683_SChhina.pdf 2.75 MB

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