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Study of sphingomyelin and ceramide model membrane phase behavior using deuterium nuclear magnetic resonance spectroscopy

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Thesis type
(Thesis) M.Sc.
Date created
Sphingomyelin is a major constituent of most eukaryotic cell plasma membranes. During apoptosis, sphingomyelin is converted into ceramide. It is hypothesized that this conversion induces a structural change in membranes that leads to downstream signaling. Deuterium nuclear magnetic resonance spectroscopy is used to create a partial phase diagram of multilamellar aqueous dispersions of palmitoyl sphingomyelin and ceramide in excess water to characterize the structural changes associated with increased ceramide content (0--40 mol$\%$ ceramide) and varying temperature (25--80\degD). The two lipids are fully miscible at high temperatures and at 40 mol$\%$ ceramide. A variety of solid-liquid coexistence phase behavior is observed at lower concentrations. A gel phase is observed at progessively higher temperatures in the sphingomyelin:ceramide membranes as ceramide content increase. This implies that at physiological temperatures, ceramide may increase the gel phase propensity of cell membranes.
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Supervisor or Senior Supervisor
Thesis advisor: Thewalt, Jenifer
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