All experiments were performed using WiCell iPS(IMR90)-1 cells, kindly gifted by Dr. G.F. Tibbits, and maintained on Matrigel® (Corning, CLS356234) coated 6-well cell culture plates in mTeSR™Plus (STEMCELL Technologies, 100-0276) following the manufacturer’s standard culturing procedures. Cells were passaged when they reached 80% confluency, approximately every 4 days: hiPSCs were dissociated to small aggregates using Versene (Gibco, 15040066) and light mechanical disruption and seeded at a split ratio of 1:20 in mTeSR™ Plus.
The supplemental data is a single PDF file. This is supplemental data to the article "Targeted activation of hERG channels rescues electrical instability induced by the hERG R56Q+/- Long QT Syndrome variant", Cardiovascular Research, cvad155, 2023.
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