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Genetic transformation and regeneration in the Galena hop variety, towards CRISPR/Cas9 modification of shoot elongation

Resource type
Thesis type
(Thesis) M.Sc.
Date created
2022-08-25
Authors/Contributors
Abstract
Hop has separate female and male individuals, and self-fertilization cannot be used to generate plants homozygous for recessive alleles and traits, inhibiting breeding efforts. A long-sought trait is semi-dwarfism, as hop bines require tall trellises and specialized harvesting equipment. Recently developed CRISPR/Cas9 genome editing technology can be used to generate homozygous recessive mutant alleles, potentially overcoming this issue, but typically requires initial plant transformation and regeneration of transgenic plants – technologies that are inefficient and variety-specific in hop. Here we demonstrate the first example of (1) genetic transformation and shoot regeneration in the popular hop cultivar Galena and (2) improved transformation in hop through the ectopic expression of Arabidopsis BABYBOOM and WUSCHEL transcription factors. In addition, targets for gene editing of semi-dwarfism were identified and CRISPR cassettes for loss of function genome editing were generated and introduced into the Galena variety. Transformants are currently being evaluated based on genotype and stature.
Document
Extent
113 pages.
Identifier
etd22120
Copyright statement
Copyright is held by the author(s).
Permissions
This thesis may be printed or downloaded for non-commercial research and scholarly purposes.
Supervisor or Senior Supervisor
Thesis advisor: Mattsson, Jim
Language
English
Member of collection
Download file Size
etd22120.pdf 10.31 MB

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