Skip to main content

Presence of an EML4-ALK Gene Fusion Detected by Microfluidic Chip DNA Hybridization

Date created
2021-01-28
Authors/Contributors
Abstract
Non-small cell lung cancer (NSCLC) accounts for ∼80-85% of all lung cancer cases, and the EML4-ALK fusion oncogene is a well-known contributor to NSCLC cases. Expensive methods such as FISH, IHC, and NGS have been used to detect the EML4-ALK fusion oncogene. Here, a cost-effective and facile method of detecting and differentiating an EML4-ALK fusion oncogene from the wild-type gene has been accomplished by DNA hybridization using the microfluidic biochip. First, oligonucleotide probes were confirmed for successful detection of immobilized sense strands. Second, capture of the sense PCR product strands (fusion and WT) and their subsequent detection and differentiation were accomplished. Our proof-of-concept study shows the ability to detect 1% fusion products, among WT ones.
Document
Scholarly level
Peer reviewed?
Yes
Language
English
Member of collection

Views & downloads - as of June 2023

Views: 11
Downloads: 0