Understanding Type IV pilus-mediated secretion in Vibrio cholerae

Author: 
Date created: 
2020-12-08
Identifier: 
etd21190
Keywords: 
Type IV pili
Type II secretion
Vibrio cholerae
Toxin coregulated pilus
Minor pilin
Exoprotein
Abstract: 

Bacterial pathogens depend on the expression of virulence factors that aid host infection. A mechanistic understanding of bacterial virulence can provide insights into novel antimicrobial targets and therapies. One virulence factor is Type IV pili (T4P), long thin filaments found on bacterial surfaces with roles in adhesion, DNA uptake and exoprotein secretion. The T4P system is closely related to the Type II secretion (T2S) system where periplasmic “pseudo-pili” exhibit a piston-like motion for exoprotein export. My research aims to understand T4P-mediated exoprotein secretion in the simple T4P system of Vibrio cholerae. I show that the exoprotein’s flexible N-terminal segment is the export signal, which may bind to minor pilin at the pilus tip for delivery across the secretin channel.

Document type: 
Thesis
Rights: 
This thesis may be printed or downloaded for non-commercial research and scholarly purposes. Copyright remains with the author.
File(s): 
Supervisor(s): 
Lisa Craig
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) M.Sc.
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