Distribution of PDLIM1 at Actin-Rich Structures Generated by Invasive and Adherent Bacterial Pathogens

Resource type
Date created
2020-10-06
Authors/Contributors
Abstract
The enteric bacterial pathogens Listeria monocytogenes (Listeria) and enteropathogenic Escherichia coli (EPEC) remodel the eukaryotic actin cytoskeleton during their disease processes. Listeria generate slender actin‐rich comet/rocket tails to move intracellularly, and later, finger‐like membrane protrusions to spread amongst host cells. EPEC remain extracellular, but generate similar actin‐rich membranous protrusions (termed pedestals) to move atop the host epithelia. These structures are crucial for disease as diarrheal (and systemic) infections are significantly abrogated during infections with mutant strains that are unable to generate the structures. The current repertoire of host components enriched within these structures is vast and diverse. In this protein catalog, we and others have found that host actin crosslinkers, such as palladin and α‐actinin‐1, are routinely exploited. To expand on this list, we set out to investigate the distribution of PDLIM1, a scaffolding protein and binding partner of palladin and α‐actinin‐1, during bacterial infections. We show that PDLIM1 localizes to the site of initial Listeria entry into cells. Following this, PDLIM1 localizes to actin filament clouds surrounding immotile bacteria, and then colocalizes with actin once the comet/rocket tails are generated. Unlike palladin or α‐actinin‐1, PDLIM1 is maintained within the actin‐rich core of membrane protrusions. Conversely, α‐actinin‐1, but not PDLIM1 (or palladin), is enriched at the membrane invagination that internalizes the Listeria‐containing membrane protrusion. We also show that PDLIM1 is a component of the EPEC pedestal core and that its recruitment is dependent on the bacterial effector Tir. Our findings highlight PDLIM1 as another protein present within pathogen‐induced actin‐rich structures.
Description
The full text of this paper will be available in October 2021 due to the embargo policies of The Anatomical Record. Contact summit@sfu.ca to enquire if the full text of the accepted manuscript can be made available to you.
Identifier
DOI: 10.1002/ar.24523
Published as
Dhanda, AS, Yang, D, Kooner, A, Guttman, JA. Distribution of PDLIM1 at actin‐rich structures generated by invasive and adherent bacterial pathogens. Anat Rec. 2020; 1–20. https://doi.org/10.1002/ar.24523.
Publication details
Publication title
Anat Rec
Document title
Distribution of PDLIM1 at Actin-Rich Structures Generated by Invasive and Adherent Bacterial Pathogens
Date
2020
First page
1
Last page
20
Publisher DOI
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Copyright is held by the author(s).
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Peer reviewed?
Yes
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