Resource type
Date created
2010-01-12
Authors/Contributors
Author: He, Yuan
Author: Macauley, Matthew S.
Author: Stubbs, Keith A.
Author: Vocadlo, David J.
Author: Davies, Gideon J.
Abstract
N-Acetylglucosamine β-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response, and transcriptional regulation. Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) using a substrate-assisted catalytic mechanism. Here we define the reaction coordinate using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.
Document
Identifier
DOI: 10.1021/ja9086769
Published as
He, Y., Macauley, M. S., Stubbs, K. A., Vocadlo, D. J., & Davies, G. J. (2010). Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase. Journal of the American Chemical Society, 132(6), 1807–1809. https://doi.org/10.1021/ja9086769.
Publication details
Publication title
Journal of the American Chemical Society
Document title
Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase
Date
2010
Volume
132
Issue
6
First page
1807
Last page
1809
Published article URL
Copyright statement
Copyright is held by the author(s).
Scholarly level
Peer reviewed?
Yes
Language
English
Member of collection
| Download file | Size |
|---|---|
| visualizing_the_reaction_coordinate_of_an_o-glcnac_hydrolase.pdf | 1.26 MB |