Mislocalization of TDP-43 in the G93A Mutant SOD1 Transgenic Mouse Model of ALS

Resource type
Date created
2009-04-18
Authors/Contributors
Abstract
Previous evidence demonstrates that TAR DNA binding protein (TDP-43) mislocalization is a key pathological feature of amyotrophic lateral sclerosis (ALS). TDP-43 normally shows nuclear localization, but in CNS tissue from patients who died with ALS this protein mislocalizes to the cytoplasm. Disease specific TDP-43 species have also been reported to include hyperphosphorylated TDP-43, as well as a C-terminal fragment. Whether these abnormal TDP-43 features are present in patients with SOD1-related familial ALS (fALS), or in mutant SOD1 over-expressing transgenic mouse models of ALS remains controversial. Here we investigate TDP-43 pathology in transgenic mice expressing the G93A mutant form of SOD1. In contrast to previous reports we observe redistribution of TDP-43 to the cytoplasm of motor neurons in mutant SOD1 transgenic mice, but this is seen only in mice having advanced disease. Furthermore, we also observe rounded TDP-43 immunoreactive inclusions associated with intense ubiquitin immunoreactivity in lumbar spinal cord at end stage disease in mSOD mice. These data indicate that TDP-43 mislocalization and ubiquitination are present in end stage mSOD mice. However, we do not observe C-terminal TDP-43 fragments nor TDP-43 hyperphosphorylated species in these end stage mSOD mice. Our findings indicate that G93A mutant SOD1 transgenic mice recapitulate some key pathological, but not all biochemical hallmarks, of TDP-43 pathology previously observed in human ALS. These studies suggest motor neuron degeneration in the mutant SOD1 transgenic mice is associated with TDP-43 histopathology.
Document
Identifier
DOI: 10.1016/j.neulet.2009.04.031
Published as
Shan, X., Vocadlo, D., & Krieger, C. (2009). Mislocalization of TDP-43 in the G93A mutant SOD1 transgenic mouse model of ALS. Neuroscience Letters, 458(2), 70–74. https://doi.org/10.1016/j.neulet.2009.04.031.
Publication title
Neuroscience Letters
Document title
Mislocalization of TDP-43 in the G93A Mutant SOD1 Transgenic Mouse Model of ALS
Date
2009
Volume
458
Issue
2
First page
70
Last page
74
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Copyright is held by the author(s).
Scholarly level
Peer reviewed?
Yes
Language