Digitized Molecular Detection on Off-the-shelf Blu-ray Discs: Upgraded Resolution and Enhanced Sensitivity

Resource type
Date created
2017-03-22
Authors/Contributors
Author: Shi, Maolin
Author: Xua, Pengtao
Abstract
Beyond the intrinsic capability of storing and archiving high-definition movies and games, off-the-shelf Blu-ray discs have been adopted for the preparation of molecular binding assays, which are subsequently read and quantitated with a standard computer drive in conjunction with the disc-quality check program. The performance of this digitized molecular detection system has been examined first with an artificial “ink assay” (an array of microsize ink dots) to define the quantitation capability and the lateral resolution; the conventional biotin-streptavidin binding assay was then tested with the Blu-ray detection platform, and the results compared with that obtained on a DVD. The upgraded lateral resolution (<100 μm) and enhanced assay performance (linear response up to 0.4 μg/mL and LOD estimated to be >< 0.1 μg/mL for the trial biotin-streptavidin system augments its potential to be adapted as a cost-effective and quantitative diagnostic tool for on-site analysis and point-of-care medical diagnosis at trace amounts.
Document
Identifier
DOI: 10.1016/j.snb.2016.11.042
Published as
Shi, M.; Weng, S. S.H.; Xu, P.; Li, X.; Yu, H.-Z. Digitized Molecular Detection on Off-the-shelf Blu-ray Discs: Upgraded Resolution and Enhanced Sensitivity. Sensors and Actuators B 242 (2017). 79–86. https://doi.org/10.1016/j.snb.2016.11.042
Publication title
Sensors and Actuators B
Document title
Digitized Molecular Detection on Off-the-shelf Blu-ray Discs: Upgraded Resolution and Enhanced Sensitivity
Date
2017
Volume
242
First page
79
Last page
86
Publisher DOI
10.1016/j.snb.2016.11.042
Copyright statement
Copyright is held by the author(s).
Scholarly level
Peer reviewed?
Yes
Language
Member of collection