Integrated Smartphone-App-Chip System for On-Site Ppb-Level Colorimetric Quantitation of Aflatoxins

Resource type
Date created
2017-07-18
Authors/Contributors
Author: Li, Xiaochun
Author: Yang, Fan
Abstract
We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high-quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app. The performance of this smartphone-based detection system was tested using both spiked and moldy corn samples; consistent results with conventional ELISA kits were obtained. The achieved detection limit (3±1 μg/kg, equivalent to ppb) and dynamic response range (0.5−250 μg/kg) meet the requested testing standards set by authorities worldwide. We envision that the integrated SPAC system promises to be a simple and accurate method of food toxin quantitation, bringing much benefit for rapid on-site screening.
Document
Identifier
DOI: 10.1021/acs.analchem.7b01379
Published as
Li, X.; Yang, F.; Wong, J. X. H.; Yu, H.-Z. Integrated smartphone-app-chip system for on-site ppb-level colorimetric quantitation of aflatoxins Analytical Chemistry, 2017, 89 (17), pp 8908–8916.
Publication title
Analytical Chemistry
Document title
Integrated Smartphone-App-Chip System for On-Site Ppb-Level Colorimetric Quantitation of Aflatoxins
Date
2017
Volume
89
Issue
17
First page
8908
Last page
8916
Copyright statement
Copyright is held by the author(s).
Scholarly level
Peer reviewed?
Yes
Language
Member of collection