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The G-quadruplex-hemin DNAzyme can tag itself with the reactive substrate biotin tyramide

Resource type
Thesis type
(Thesis) M.Sc.
Date created
2017-04-19
Authors/Contributors
Abstract
Genomic database searches suggest that there are a large number of potentially G-quadruplex forming sequences present in the human genome, in addition to their well-established localization in telomeres. Novel imaging techniques support these data and have begun to indicate the extent to which G-quadruplexes are present in vivo. Information on any biological function of these sequences is less clear. This thesis presents the creation of an assay that could target G-quadruplexes for imaging or pulldown and potentially give information on their biological relevance. We utilize the inherent peroxidase activity of the quadruplex-hemin interaction to initiate a tagging reaction based upon a reactive tyramide substrate.
Document
Identifier
etd10143
Copyright statement
Copyright is held by the author.
Permissions
This thesis may be printed or downloaded for non-commercial research and scholarly purposes.
Scholarly level
Supervisor or Senior Supervisor
Thesis advisor: Sen, Dipankar
Member of collection
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etd10143_OEinarson.pdf 4.73 MB

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