Method Comparison for Analyzing Wound Healing Rates

Resource type
Date created
2017-01
Authors/Contributors
Abstract
Wound healing scratch assay is a frequently used method to characterize cell migration, which is an important biological process in the course of development, tissue repair, and immune response for example. The measurement of wound healing rate, however, varies among different studies. Here we summarized these measurements into three types: I) Direct Rate Average; II) Regression Rate Average; and III) Average Distance Regression Rate. Using Chinese Hamster Ovary (CHO) cells as a model, we compared the three types of analyses on quantifying the wound closing rate, and discovered that type I & III measurements are more resistant to outliers and type II analysis is more sensitive to outliers. We hope this study can help researchers to better use this simple yet effective assay.
Document
Published as
Dhillon, P.K., Li, X., Sanes, J.T., Akintola, O.S., and Sun, B.(2017). Method Comparison for Analyzing Wound Healing Rates. Biochemistry and Cell Biology (in press). http://dx.doi.org/10.1139/bcb-2016-0163
Publication title
Biochemistry and Cell Biology
Document title
Method Comparison for Analyzing Wound Healing Rates
Date
2017
Publisher DOI
10.1139/bcb-2016-0163
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Copyright is held by the author(s).
Scholarly level
Peer reviewed?
Yes
Language
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bcb-2016-0163.pdf 573.45 KB