A microfluidic antibody bioarray for detection of human interleukins

Date created: 
2016-12-16
Identifier: 
etd9953
Keywords: 
Antibody bioarray
Polydimethylsiloxane (PDMS)
Microfluidics
Antibody immobilization
Antibody-antigen interaction
Sandwich Enzyme linked immunosorbent assay (ELISA)
Abstract: 

The purpose of the present work is to investigate the factors affecting antibody immobilization, and antibody-antigen interactions on a microfluidic chip. The results of this study will be utilized for the development of a microfluidic antibody bioarray for detection of two target proteins. Two interleukins of diagnostic value have been selected: Interleukin-6 (IL-6), and Interleukin-2 (IL-2). The micromosaic array is used for detection of IL-2 and IL-6 on a microfluidic chip. This method is used to optimize a variety of factors that affect antibody immobilization on the surface of a microfluidic chip, as well as bioarray conditions for enhancement of signals. Surface Plasmon Resonance (SPR) spectroscopy is used to obtain the association and dissociation rate constants for antibody-antigen binding in this work.

Document type: 
Thesis
Rights: 
This thesis may be printed or downloaded for non-commercial research and scholarly purposes. Copyright remains with the author.
File(s): 
Supervisor(s): 
Paul Li
Department: 
Science: Department of Chemistry
Thesis type: 
(Thesis) M.Sc.
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