A microfluidic antibody bioarray for detection of human interleukins

The purpose of the present work is to investigate the factors affecting antibody immobilization, and antibody-antigen interactions on a microfluidic chip. The results of this study will be utilized for the development of a microfluidic antibody bioarray for detection of two target proteins. Two interleukins of diagnostic value have been selected: Interleukin-6 (IL-6), and Interleukin-2 (IL-2). The micromosaic array is used for detection of IL-2 and IL-6 on a microfluidic chip. This method is used to optimize a variety of factors that affect antibody immobilization on the surface of a microfluidic chip, as well as bioarray conditions for enhancement of signals. Surface Plasmon Resonance (SPR) spectroscopy is used to obtain the association and dissociation rate constants for antibody-antigen binding in this work.