Whole-Genome Sequencing Of Mesorhizobium huakuii 7653R Provides Molecular Insights into Host Specificity and Symbiosis Island Dynamics

Resource type
Date created
2014
Authors/Contributors
Author: Hao, Baohai
Author: Li, Jiarui
Author: Gu, Huilin
Author: Peng, Jieli
Author: Xie, Fuli
Author: Zhao, Xinyin
Author: Ma, Binguang
Author: Li, Youguo
Abstract
BackgroundEvidence based on genomic sequences is urgently needed to confirm the phylogenetic relationship between Mesorhizobium strain MAFF303099 and M. huakuii. To define underlying causes for the rather striking difference in host specificity between M. huakuii strain 7653R and MAFF303099, several probable determinants also require comparison at the genomic level. An improved understanding of mobile genetic elements that can be integrated into the main chromosomes of Mesorhizobium to form genomic islands would enrich our knowledge of how genome dynamics may contribute to Mesorhizobium evolution in general.ResultsIn this study, we sequenced the complete genome of 7653R and compared it with five other Mesorhizobium genomes. Genomes of 7653R and MAFF303099 were found to share a large set of orthologs and, most importantly, a conserved chromosomal backbone and even larger perfectly conserved synteny blocks. We also identified candidate molecular differences responsible for the different host specificities of these two strains. Finally, we reconstructed an ancestral Mesorhizobium genomic island that has evolved into diverse forms in different Mesorhizobium species.ConclusionsOur ortholog and synteny analyses firmly establish MAFF303099 as a strain of M. huakuii. Differences in nodulation factors and secretion systems T3SS, T4SS, and T6SS may be responsible for the unique host specificities of 7653R and MAFF303099 strains. The plasmids of 7653R may have arisen by excision of the original genomic island from the 7653R chromosome.
Document
Published as
BMC Genomics 2014, 15:440 doi:10.1186/1471-2164-15-440The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2164/15/440
Publication title
BMC Genomics
Document title
Whole-Genome Sequencing Of Mesorhizobium huakuii 7653R Provides Molecular Insights into Host Specificity and Symbiosis Island Dynamics
Date
2014
Volume
15
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Copyright is held by the author(s).
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Peer reviewed?
Yes
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1471-2164-15-440.pdf 2.79 MB