Investigating the localization and function of the C. elegans HAM-1 protein

Author: 
Date created: 
2014-12-02
Identifier: 
etd8727
Keywords: 
C. elegans
Asymmetric cell division
HAM-1
Abstract: 

In C. elegans, HAM-1 is an asymmetrically localized protein that regulates several asymmetric neuroblast divisions. Although the protein contains a putative DNA binding domain, nuclear localization of HAM-1 has never been observed through immunostaining of embryos with anti-HAM-1 antibodies. However, a GFP::HAM-1 fusion protein has been detected in the nucleus of transgenic animals under direct fluorescence microscopy. Through a biochemical subcellular fractionation of embryonic extracts, I determined that endogenous HAM-1 is primarily a nuclear protein. I have also examined HAM-1 localization in different ham-1 mutant embryos. This analysis has revealed several key residues in the N-terminus and a region in the C-terminus that are required for proper cortical localization of HAM-1. Finally, I have shown that Tyrosine residue 369 is crucial for HAM-1 function. Thus, my work has lent insight into HAM-1 sequences that contribute to function and localization of the protein.

Document type: 
Thesis
Rights: 
Copyright remains with the author. The author granted permission for the file to be printed and for the text to be copied and pasted.
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Supervisor(s): 
Nancy Hawkins
Department: 
Science:
Thesis type: 
(Thesis) M.Sc.
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