Resource type
Date created
2013
Authors/Contributors
Author: Mwimanzi, Philip
Author: Markle, Tristan
Author: Martin, Eric
Author: Ogata, Yoko
Author: Kuang, Xiaomei
Author: Tokunaga, Michiyo
Author: Mahiti, Macdonald
Author: Pereyra, Florencia
Author: Miura, Toshiyuki
Author: Walker, Bruce
Author: Brumme, Zabrina
Author: Brockman, Mark
Author: Ueno, Takamasa
Abstract
BackgroundImpaired HIV-1 Gag, Pol, and Env function has been described in elite controllers (EC) who spontaneously suppress plasma viremia to < 50 RNA copies/mL; however, activity of the accessory protein Nef remains incompletely characterized. We examined the ability of 91 Nef clones, isolated from plasma of 45 EC and 46 chronic progressors (CP), to down-regulate HLA class I and CD4, up-regulate HLA class II invariant chain (CD74), enhance viral infectivity, and stimulate viral replication in PBMC.ResultsIn general, EC Nef clones were functional; however, all five activities were significantly lower in EC compared to CP. Nef clones from HLA-B*57-expressing EC exhibited poorer CD4 down-regulation function compared to those from non-B*57 EC, and the number of EC-specific B*57-associated Nef polymorphisms correlated inversely with 4 of 5 Nef functions in these individuals.ConclusionResults indicate that decreased HIV-1 Nef function, due in part to host immune selection pressures, may be a hallmark of the EC phenotype.
Document
Published as
Retrovirology 2013, 10:1 doi:10.1186/1742-4690-10-1
Publication details
Publication title
Retrovirology
Document title
Attenuation Of Multiple Nef Functions In HIV-1 Elite Controllers
Date
2013
Volume
10
Issue
1
Publisher DOI
10.1186/1742-4690-10-1
Rights (standard)
Copyright statement
Copyright is held by the author(s).
Scholarly level
Peer reviewed?
Yes
Language
English
Member of collection
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