Prevention of myocardial infarctions, using non-invasive biophotonics measurement of biomarker cardiac troponin I.

Coronary Heart Disease is the leading cause of death worldwide. Myocardial Infarctions are one of the most frequent cardiovascular diseases. This research aims to design a non-invasive heart attack detection system based on biphotonic measurements. Several methods such as ECG monitoring, Florescence and Raman Spectroscopy are evaluated and Raman Spectroscopy is chosen due to advantages in sensitivity and efficiency. Since cTnI test is the current gold standard in diagnosing MI, the initial part of this research is based on comprehensive study of this protein. In this research Raman spectra of cTnI is measured and after assessment of possible adjustments to noise level, SERS method is used to obtain the enhanced spectra and consequently a unique cTnI Raman signature. Due to low biological concentrations and low SNR, the spectra are measured in higher concentrations. Finally, a conceptual Raman design is proposed as a part of the instrumentation study of the system.