High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans

Resource type
Date created
2007-09
Authors/Contributors
Author: Mah, Allan
Author: Fang, Lily
Author: Lee, David
Author: Lin, John
Author: Lorch, Adam
Author: Pan, Jie
Author: Tu, Domena
Author: Wong, Kim
Author: Zhao, Z.
Abstract
Using DNA sequences 5′ to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism. GFP-expressing wild-type animals were analyzed at each stage of development from embryo to adult. We have identified 5′ DNA regions regulating expression at all developmental stages and in 38 different cell and tissue types in this organism. Among the regulatory regions identified are sequences that regulate expression in all cells, in specific tissues, in combinations of tissues, and in single cells. Most of the genes we have examined in C. elegans have human orthologs. All the images and expression pattern data generated by this project are available at WormAtlas (http://gfpweb.aecom.yu.edu/index) and through WormBase (http://www.wormbase.org).
Document
Published as
Hunt-Newbury R, Viveiros R, Johnsen R, Mah A, Anastas D, et al. (2007) High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans . PLoS Biol 5(9): e237. doi:10.1371/journal.pbio.0050237
Publication title
PLoS Biol
Document title
High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans
Date
2007
Volume
5
Issue
9
Publisher DOI
10.1371/journal.pbio.0050237
Copyright statement
Copyright is held by the author(s).
Scholarly level
Peer reviewed?
Yes
Funder
Funder: Genome BC
Language
Member of collection
Attachment Size
Hun-Newberry2007.pdf 1.22 MB