Author: Wong, Tammy
The emergence of Next Generation DNA Sequencing (NGS) technologies has made cloning of genes much more efficient by dramatically reducing the cost, labour and time required. In this work, I have applied a NGS and devised a bioinformatics program pipeline to detect genomics alterations in the mutant strains of the nematode Caenorhabditis elegans. In the first project, I have detected a mutation in tba-5, validated through complementation tests, and proposed a model elucidating the role of TBA-5 in cilia structure. In the second project, the variant detection pipeline is used to isolate potential candidate genes corresponding to various dsh-2(or302) suppressors, namely Sup327, Sup245 and Sup305. Additionally, I have applied the bioinformatics pipeline to confirm that the current C. briggsae reference genome harbours thousands of assembly errors, many of which affect the correct prediction of gene models.
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Thesis advisor: Chen, Jack
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