Pregnancy-associated plasma protein A2 (PAPPA2) expression in the placenta

Resource type
Thesis type
(Thesis) M.Sc.
Date created
2011-03-29
Authors/Contributors
Abstract
Pregnancy-associated plasma protein A2 (PAPPA2), an insulin-like growth factor binding protein-5 (IGFBP-5) protease, has been shown to be upregulated during pre-eclampsia, a serious hypertensive disorder that occurs during pregnancy. The goal of this thesis was to study placental PAPPA2 expression using mice and cell culture models. In mice with natural genetic variation at the PAPPA2 gene, there was large variation in placental PAPPA2 expression, but this did not alter pregnancy outcomes, such as birth weight. In order to study potential factors regulating PAPPA2 expression, the human choriocarcinoma cell line, BeWo, was used as a model of placental trophoblasts. Hypoxia, prostaglandin E2, and tumor necrosis factor-alpha (factors present at elevated levels in pre-eclampsia) all caused PAPPA2 upregulation. Both the mouse and cell culture results are consistent with the hypothesis that PAPPA2 is upregulated in response to placental pathology, rather than being a cause of pregnancy complications.
Document
Identifier
etd6519
Copyright statement
Copyright is held by the author.
Permissions
The author granted permission for the file to be printed and for the text to be copied and pasted.
Scholarly level
Supervisor or Senior Supervisor
Thesis advisor: Christians, Julian
Member of collection
Attachment Size
etd6519_PWagner.pdf 658.62 KB