Resource type
Thesis type
(Thesis) M.Sc.
Date created
2010-12-09
Authors/Contributors
Author (aut): Lau, Edward Gee Ming
Abstract
Proteins are primarily responsible for functionalizing cells and, with respect to an initiative in soft ionization mass spectrometry (MS), the relative abundances of selected proteins have been used as indicators of normal or stressed physiological states. Methods developed for this thesis describe the use of matrix assisted laser desorption ionization time of flight (MALDI-ToF) MS to monitor the ion signals of C-X-C motif chemokine 5 and ubiquitin protein. Identification of these ion signals were carried out by performing additional experiments using liquid chromatography interfaced to an electrospray ionization (LC-ESI) equipped MS capable of tandem MS. A total of 78 proteins were identified by LC-ESI tandem MS, but not all corresponded to MALDI-MS data. In addition to the use of commercially available instrumentation, a separate study was performed to investigate the potential for an AC trap to generate molecular ions from a single levitated droplet having undergone Coulomb explosion.
Document
Identifier
etd6396
Copyright statement
Copyright is held by the author.
Scholarly level
Supervisor or Senior Supervisor
Thesis advisor (ths): Agnes, George
Member of collection
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