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Software based bioassay quantization using standard optical disc drives

Resource type
Thesis type
(Thesis) M.A.Sc.
Date created
2009-12-17
Authors/Contributors
Abstract
This thesis describes the application of the compact disc technology as a rapid, low-cost, high capacity-screening platform for bioassays. Although the compact disc has been used by modifying the hardware of the detection mechanism (CD-drive) to achieve a ubiquitous and low-cost detection of biomolecules, minimal or no modification is ideal. This thesis investigates the possibility of an inexpensive, software based bioassay detection on CDs with, standard computer drives. Using a CD data analysis software called IsoBuster, biotin-streptavidin binding assays prepared on compact discs are read detected and quantized using error based detection mechanism with a high spatial accuracy. The research shows a sensitivity of detection of 270 μg/mL of streptavidin in solution with biotin-modified disc
Document
Identifier
etd6229
Copyright statement
Copyright is held by the author.
Permissions
The author granted permission for the file to be printed and for the text to be copied and pasted.
Scholarly level
Supervisor or Senior Supervisor
Thesis advisor: Parameswaran, Ash
Member of collection
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etd6229_MPallapa.pdf 3.97 MB

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