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Light-controlled regulation of eukaryotic gene expression using a diffusible effector

Resource type
Thesis type
(Thesis) M.Sc.
Date created
2010-07-28
Authors/Contributors
Abstract
This project focuses on the design of a small synthetic, trans-acting noncoding RNA to directly regulate gene expression by light irradiation in a eukaryotic system at the mRNA level. This trans-acting noncoding RNA is designed to incorporate an in vitro-selected small ligand-binding domain, called an aptamer domain. This aptamer domain selectively binds to one but not the other of the two isomers of a dihydropyrene photo-switch compound. Such an aptamer sequence allowed us to design an allosteric riboregulator, a RNA molecule with a regulatory function whose mode of activity depends on its photo-switch ligand binding to the aptamer domain, which in turn is influenced by light irradiation at an appropriate wavelength. This light-controlled riboregulator is referred to as an antiswitch. This antiswitch is specifically designed to control the expression of Green Fluorescent protein (GFP) in vivo in Saccharomyces cerevisiae as a model organism.
Document
Identifier
etd6158
Copyright statement
Copyright is held by the author.
Permissions
The author granted permission for the file to be printed, but not for the text to be copied and pasted.
Scholarly level
Supervisor or Senior Supervisor
Thesis advisor: Sen, Dipankar
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etd6158_NAzrahimi.pdf 1.83 MB

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