The effects of a high-fat diet and bacterial lipopolysaccharide (LPS) induced inflammation on pregnancy and fetal development in mice

Author: 
Date created: 
2020-06-30
Identifier: 
etd20941
Keywords: 
Pregnancy
Inflammation
High-fat diet
Lipopolysaccharide
Abstract: 

Inflammation during pregnancy can disturb maternal tolerance of the fetus. In mice, maternal high-fat diet (HFD) induces inflammation without pregnancy complications. I hypothesised that an additional inflammatory insult would exacerbate the immune response, leading to serious complications. To test this, I developed a HFD/LPS model, where female mice were fed a high-fat or low-fat diet prior to mating, and then treated with either bacterial lipopolysaccharide (LPS), an inflammatory stimulant, or a control. Diet, LPS or a diet-LPS interaction had no effect on fetal and placental parameters or maternal levels of TNF-α, an inflammatory marker (p>0.05). Furthermore, fetal and placental parameters did not differ between HFD mice that were prone or resistant to weight-gain. While diet or a diet-LPS interaction did not affect pregnancy, LPS treatment alone caused complete fetal loss in some mice (p<0.05). These findings suggest that LPS does not exacerbate the inflammatory effects of HFD in pregnant mice.

Document type: 
Thesis
Rights: 
This thesis may be printed or downloaded for non-commercial research and scholarly purposes. Copyright remains with the author.
File(s): 
Supervisor(s): 
Julian Christians
Department: 
Science: Biological Sciences Department
Thesis type: 
(Thesis) M.Sc.
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