A Selective Inhibitor Gal‐PUGNAc of Human Lysosomal β‐Hexosaminidases Modulates Levels of the Ganglioside GM2 in Neuroblastoma Cells

Peer reviewed: 
Yes, item is peer reviewed.
Scholarly level: 
Faculty/Staff
Final version published as: 

Stubbs, K. A., Macauley, M. S., & Vocadlo, D. J. (2009). A Selective Inhibitor Gal-PUGNAc of Human Lysosomal β-Hexosaminidases Modulates Levels of the Ganglioside GM2 in Neuroblastoma Cells. Angewandte Chemie International Edition, 48(7), 1300–1303. https://doi.org/10.1002/anie.200804583.

Date created: 
2009-01-28
Identifier: 
DOI: 10.1002/anie.200804583
Keywords: 
Carbohydrates
Gangliosides
Glycosidases
Inhibitors
Abstract: 

Gal‐PUGNAc (see picture), a highly selective inhibitor for β‐hexosaminidases HEXA and HEXB is cell‐permeable and modulates the activity of HEXA and HEXB in tissue culture, increasing ganglioside GM2 levels. Gal‐PUGNAc should allow the role of these enzymes to be studied at the cellular level without generating a complex chemical phenotype from concomitant inhibition of O‐GlcNAcase.

Language: 
English
Document type: 
Article
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Rights remain with the authors.
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Sponsor(s): 
Natural Sciences and Engineering Research Council of Canada (NSERC)
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