Cyclophilin A Controls Salmonella Internalization Levels and is Present at E. coli Actin‐Rich Pedestals

Peer reviewed: 
Yes, item is peer reviewed.
Scholarly level: 
Graduate student (PhD)
Final version published as: 

Dhanda, A.S., Warren, K.E., Chiu, R.H. and Guttman, J.A. (2018), Cyclophilin A Controls Salmonella Internalization Levels and is Present at E. coli Actin‐Rich Pedestals. Anat Rec, 301: 2086-2094. https://doi.org/10.1002/ar.23957

Date created: 
2018-10-12
Identifier: 
DOI: 10.1002/ar.23957
Keywords: 
S. Typhimurium
EHEC
EPEC
Cyclophilin A
Membrane ruffles
Pedestals
Abstract: 

Salmonella enterica serovar Typhimurium (S. Typhimurium), enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) commandeer the actin cytoskeleton of their host cells as a crucial step in their infectious processes. These pathogens depend on the injection of their own effectors directly into target host cells in order to usurp cellular signaling pathways that lead to morphological actin rearrangements in those cells. Here we show that the PPIase Cyclophilin A (CypA) is a novel component of S. Typhimurium-induced membrane ruffles and functions to restrict bacterial invasion levels, as in cells depleted of CypA, bacterial loads increase. We also demonstrate that CypA requires the EPEC effector Tir as well as pedestal formation for its recruitment to bacterial attachment sites and that its presence at pedestals also holds during EHEC infections. Finally, we demonstrate that CypA is found at lamellipodia; actin-rich structures at the leading edge of motile cells. Our findings further establish CypA as a component of dynamic actin-rich structures formed during bacterial infections and within cells in general.

Language: 
English
Document type: 
Article
Rights: 
Rights remain with the authors.
File(s): 
Statistics: