Voltage-gated sodium channels and their role in prostate cancer

Author: 
Date created: 
2019-12-09
Identifier: 
etd20692
Keywords: 
Voltage-gated sodium channels
Cancer
Immunocytochemistry
Invasion assays
Patch clamping
Cell invasion
Abstract: 

Voltage-gated sodium channels (VGSC) increase invasiveness and metastatic potential in prostate cancer and may be a novel drug target for castration resistant prostate cancer. VGSC isoforms expressed in prostate cancer differ depending on cell type, and laboratory growing conditions Immunocytochemistry experiments suggest that VGSC localize in invadopodia, structures required for invasiveness of cancer cells. VGSC significantly colocalize with vimentin, a marker of invadopodia (p<0.0001) and display polarized expression patterns on the cell membrane. Functional invasion experiments using a variety of VGSC blockers such as tetrodotoxin, lidocaine, and cannabidiol demonstrate that VGSC inhibition significantly reduces cancer invasiveness (p<0.0001). These results suggest that VGSC plays a functional role in invadopodia and VGSC inhibition reduces invasiveness and metastatic potential in prostate cancer.

Document type: 
Thesis
Rights: 
This thesis may be printed or downloaded for non-commercial research and scholarly purposes. Copyright remains with the author.
File(s): 
Supervisor(s): 
Dr. Peter Ruben
Department: 
Science: Department of Biomedical Physiology and Kinesiology
Thesis type: 
(Thesis) M.Sc.
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