Bacteria-delivered RNA interference strategies to silence genes related to vector competence of Aedes aegypti

Author: 
Date created: 
2018-05-29
Identifier: 
etd10734
Keywords: 
Aedes aegypti
Dengue
Bacteria-delivered dsRNA
Cathepsin B
Fitness test
Antibiotic treatment
Abstract: 

Dengue infection is a devastating mosquito-borne disease, and the principal vector is the mosquito, Aedes aegypti. Current vector control strategies are not working, hence the need for alternative strategies. Cathepsin B is a mosquito protein that dengue viruses require to establish and replicate within mosquitoes; knocking down cathepsin B using RNAi changes the phenotype from dengue susceptible to dengue refractory in Ae. aegypti. We engineered bacteria to express dsRNA against cathepsin B to develop an orally delivered RNAi system. Our data suggest inconsistencies in the alteration of gene expression that may be a result of the modified bacteria being digested, or a lower than required quantity of the RNAi constructs being expressed. Without a consistent knockdown, it is unlikely that we will be able to reduce vector competence predictably.

Document type: 
Thesis
Rights: 
This thesis may be printed or downloaded for non-commercial research and scholarly purposes. Copyright remains with the author.
File(s): 
Senior supervisor: 
Carl Lowenberger
Department: 
Science: Biological Sciences Department
Thesis type: 
(Thesis) M.P.M.
Statistics: