Digitized Molecular Detection on Off-the-shelf Blu-ray Discs: Upgraded Resolution and Enhanced Sensitivity

Peer reviewed: 
Yes, item is peer reviewed.
Scholarly level: 
Faculty/Staff
Final version published as: 

Shi, M.; Weng, S. S.H.; Xu, P.; Li, X.; Yu, H.-Z. Digitized Molecular Detection on Off-the-shelf Blu-ray Discs: Upgraded Resolution and Enhanced Sensitivity. Sensors and Actuators B 242 (2017). 79–86.  https://doi.org/10.1016/j.snb.2016.11.042

Date created: 
2017-03-22
Identifier: 
DOI: 10.1016/j.snb.2016.11.042
Keywords: 
Blu-ray disc
Optical drive
Error correction
Molecular detection
Bioassay
Abstract: 

Beyond the intrinsic capability of storing and archiving high-definition movies and games, off-the-shelf Blu-ray discs have been adopted for the preparation of molecular binding assays, which are subsequently read and quantitated with a standard computer drive in conjunction with the disc-quality check program. The performance of this digitized molecular detection system has been examined first with an artificial “ink assay” (an array of microsize ink dots) to define the quantitation capability and the lateral resolution; the conventional biotin-streptavidin binding assay was then tested with the Blu-ray detection platform, and the results compared with that obtained on a DVD. The upgraded lateral resolution (<100 μm) and enhanced assay performance (linear response up to 0.4 μg/mL and LOD estimated to be >< 0.1 μg/mL for the trial biotin-streptavidin system augments its potential to be adapted as a cost-effective and quantitative diagnostic tool for on-site analysis and point-of-care medical diagnosis at trace amounts.

Description: 

The full text of this paper will be available on April 2, 2018 in accordance with the embargo periods of Elsevier journals for NSERC funded research. If you require a fulltext copy of this paper before April 2018 please contact summit@sfu.ca

Language: 
English
Document type: 
Article
Rights: 
Rights remain with the authors.
Sponsor(s): 
Natural Sciences and Engineering Research Council of Canada (NSERC)
Natural Science Foundation of China
China Scholarship Council
Statistics: