Integrated Smartphone-App-Chip System for On-Site Ppb-Level Colorimetric Quantitation of Aflatoxins

Peer reviewed: 
Yes, item is peer reviewed.
Scholarly level: 
Faculty/Staff
Final version published as: 

Li, X.; Yang, F.; Wong, J. X. H.; Yu, H.-Z. Integrated smartphone-app-chip system for on-site ppb-level colorimetric quantitation of aflatoxins Analytical Chemistry, 2017, 89 (17), pp 8908–8916.

Date created: 
2017-07-18
Identifier: 
DOI: 10.1021/acs.analchem.7b01379
Keywords: 
Aflatoxins
Smartphone
Mobile app
Microchip
On-site chemical analysis
Colorimetric quantitation
Abstract: 

We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high-quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app. The performance of this smartphone-based detection system was tested using both spiked and moldy corn samples; consistent results with conventional ELISA kits were obtained. The achieved detection limit (3±1 μg/kg, equivalent to ppb) and dynamic response range (0.5−250 μg/kg) meet the requested testing standards set by authorities worldwide. We envision that the integrated SPAC system promises to be a simple and accurate method of food toxin quantitation, bringing much benefit for rapid on-site screening.

Language: 
English
Document type: 
Article
Rights: 
Rights remain with the authors.
File(s): 
Sponsor(s): 
Natural Sciences and Engineering Research Council of Canada (NSERC)
Natural Science Foundation of China
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