Integrated Smartphone-App-Chip System for On-Site Ppb-Level Colorimetric Quantitation of Aflatoxins

Peer reviewed: 
Yes, item is peer reviewed.
Scholarly level: 
Faculty/Staff
Final version published as: 

Li, X.; Yang, F.; Wong, J. X. H.; Yu, H.-Z. Integrated smartphone-app-chip system for on-site ppb-level colorimetric quantitation of aflatoxins Analytical Chemistry, 2017, 89 (17), pp 8908–8916.

Date created: 
2017-07-18
Keywords: 
Aflatoxins
Smartphone
Mobile app
Microchip
On-site chemical analysis
Colorimetric quantitation
Abstract: 

We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high-quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app. The performance of this smartphone-based detection system was tested using both spiked and moldy corn samples; consistent results with conventional ELISA kits were obtained. The achieved detection limit (3±1 μg/kg, equivalent to ppb) and dynamic response range (0.5−250 μg/kg) meet the requested testing standards set by authorities worldwide. We envision that the integrated SPAC system promises to be a simple and accurate method of food toxin quantitation, bringing much benefit for rapid on-site screening.

Description: 

The full text of this paper will be available on August 1, 2018 in accordance with the embargo periods of the American Chemical Society and in keeping with the Open Access requirements of NSERC funded research. If you require a fulltext copy of this paper before August 2018 please contact summit@sfu.ca.

Language: 
English
Document type: 
Article
Rights: 
Rights remain with the authors.
Sponsor(s): 
Natural Sciences and Engineering Research Council of Canada (NSERC)
Natural Science Foundation of China
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