The G-quadruplex-hemin DNAzyme can tag itself with the reactive substrate biotin tyramide

Date created: 
2017-04-19
Identifier: 
etd10143
Keywords: 
G-quadruplex
Peroxidase
DNAzyme
Aptamer
Tyramide
Abstract: 

Genomic database searches suggest that there are a large number of potentially G-quadruplex forming sequences present in the human genome, in addition to their well-established localization in telomeres. Novel imaging techniques support these data and have begun to indicate the extent to which G-quadruplexes are present in vivo. Information on any biological function of these sequences is less clear. This thesis presents the creation of an assay that could target G-quadruplexes for imaging or pulldown and potentially give information on their biological relevance. We utilize the inherent peroxidase activity of the quadruplex-hemin interaction to initiate a tagging reaction based upon a reactive tyramide substrate.

Document type: 
Thesis
Rights: 
This thesis may be printed or downloaded for non-commercial research and scholarly purposes. Copyright remains with the author.
File(s): 
Senior supervisor: 
Dipankar Sen
Department: 
Science: Department of Chemistry
Thesis type: 
(Thesis) M.Sc.
Statistics: