Trypanosomes Modify the Behavior of Their Insect Hosts: Effects on Locomotion and on the Expression of a Related Gene

Peer reviewed: 
Yes, item is peer reviewed.
Scholarly level: 
Faculty/Staff
Final version published as: 

Marliére NP, Latorre-Estivalis JM, Lorenzo MG, Carrasco D, Alves-Silva J, Rodrigues JdO, et al. (2015) Trypanosomes Modify the Behavior of Their Insect Hosts: Effects on Locomotion and on the Expression of a Related Gene. PLoS Negl Trop Dis 9(8): e0003973. doi:10.1371/journal.pntd.0003973

Date created: 
2015
Keywords: 
Insects
Biological locomotion
Trypanosoma cruzi
Insect vectors
Nymphs
Foraging
Host-pathogen interactions
Animal behavior
Abstract: 

Background

As a result of evolution, the biology of triatomines must have been significantly adapted to accommodate trypanosome infection in a complex network of vector-vertebrate-parasite interactions. Arthropod-borne parasites have probably developed mechanisms, largely still unknown, to exploit the vector-vertebrate host interactions to ensure their transmission to suitable hosts. Triatomines exhibit a strong negative phototaxis and nocturnal activity, believed to be important for insect survival against its predators.

Methodology/Principal Findings

In this study we quantified phototaxis and locomotion in starved fifth instar nymphs of Rhodnius prolixus infected with Trypanosoma cruzi or Trypanosoma rangeli. T. cruzi infection did not alter insect phototaxis, but induced an overall 20% decrease in the number of bug locomotory events. Furthermore, the significant differences induced by this parasite were concentrated at the beginning of the scotophase. Conversely, T. rangeli modified both behaviors, as it significantly decreased bug negative phototaxis, while it induced a 23% increase in the number of locomotory events in infected bugs. In this case, the significant effects were observed during the photophase. We also investigated the expression of Rpfor, the triatomine ortholog of the foraging gene known to modulate locomotion in other insects, and found a 4.8 fold increase for T. rangeli infected insects.

Conclusions/Significance

We demonstrated for the first time that trypanosome infection modulates the locomotory activity of the invertebrate host. T. rangeli infection seems to be more broadly effective, as besides affecting the intensity of locomotion this parasite also diminished negative phototaxis and the expression of a behavior-associated gene in the triatomine vector.

Language: 
English
Document type: 
Article
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Sponsor(s): 
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
Programa Estratégico de Apoio a Pesquisa em Saúde VI/FIOCRUZ
Programa de Excelência em Pesquisa
Natural Sciences and Engineering Research Council of Canada (NSERC)
Canada Research Chairs program
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