Refolding Recombinant Escherichia coli BamA: the integral membrane component of the BAM (Beta-barrel Assembly Machinery) complex

Author: 
Date created: 
2014-07-23
Identifier: 
etd8598
Keywords: 
Outer membrane
Beta-barrel outer membrane protein
Beta-barrel assembly machinery complex
Protein refolding
Abstract: 

The selective permeability of the outer membrane (OM) of Gram-negative bacteria is mainly determined by the pore-forming proteins within it, which are called outer membrane proteins (OMPs). The proteinaceous apparatus required to fold and assemble the OMPs is known as the beta-barrel assembly machinery (BAM) complex. In Escherichia coli, the BAM complex is made up of five proteins. BamA is a beta-barrel protein integrated into the OM, while BamB, BamC, BamD and BamE are lipoproteins attached to the periplasmic side of the OM by a covalently bound lipid. Over-expression of BamA without its signal peptide results in insoluble inclusion body formation. I have investigated refolding strategies for E. coli BamA. The refolded BamA has been analysed by size-exclusive chromatography, heat-modifiability, circular dichroism spectroscopy and limited proteolysis. The experimental results are consistent with both a full length BamA and a truncated BamA construct being refolded properly using my discovered conditions. Both refolded constructs are able to crystallize. These initial crystallization conditions will be helpful in the structural analysis of E. coli BamA.

Document type: 
Thesis
Rights: 
Copyright remains with the author. The author granted permission for the file to be printed and for the text to be copied and pasted.
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Supervisor(s): 
Mark Paetzel
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) M.Sc.
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