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Fatty acid binding proteins in the fall armyworm, Spodoptera frugiperda

Resource type
Thesis type
(Thesis) M.Sc.
Date created
2013-07-26
Authors/Contributors
Author: Jiang, Xin
Abstract
The expression of fatty acid binding protein (FABP) is often up-regulated during high energy demanding activities. In this study, we have confirmed the expression of two FABPs in the flight muscles of the fall armyworm, Spodoptera frugiperda. Based on real-time PCR results, the expression of both genes appeared to be affected by flight. The promoter of one gene was sequenced and cloned into a GFP reporter vector for a reporter assay using the SF9 cell line. The expression of this gene in the SF9 cell line does not appear to be up-regulated in response to fatty acid treatment. Therefore, alternative strategies to analyze the regulation of this gene are required.
Document
Identifier
etd8027
Copyright statement
Copyright is held by the author.
Permissions
The author has not granted permission for the file to be printed nor for the text to be copied and pasted. If you would like a printable copy of this thesis, please contact summit-permissions@sfu.ca.
Scholarly level
Supervisor or Senior Supervisor
Thesis advisor: Haunerland, Norbert
Language
English
Member of collection
Download file Size
ETD8027_XJ.pdf 3.08 MB

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