Molecular Biology and Biochemistry - Theses, Dissertations, and other Required Graduate Degree Essays

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Identification, evolution and expression of three olfactory gene families in Atlantic salmon (Salmo salar)

Date created: 
2011-11-30
Abstract: 

It has been hypothesized that salmonids use olfactory cues to return to their natal rivers and streams. However, the key components of the molecular pathway involved in imprinting and homing are still unknown. If odorants are involved in salmon homing migration then olfactory receptors should play a critical role in the dissipation of information from the environment to the fish. To understand the molecular basis for imprinting and homing in Atlantic salmon (Salmo salar), it is important to identify and characterize the olfactory receptors in the Atlantic salmon genome. Aquatic chemical cues are received through the salmon nares and into the nasal cavity that contains a single olfactory organ, olfactory rosette. The olfactory rosette contains sensory neurons, which are thought to express only one olfactory receptor. In this study, three major superfamilies of fish olfactory receptors (MOR, ora and OlfC) were examined. To identify the olfactory genes in Atlantic salmon several genomic and bioinformatic techniques were used. First, an Atlantic salmon bacterial artificial chromosome (BAC) library was screened with probes designed from previously identified fish olfactory receptor sequences. Then a selected number of hybridization positive BACs containing olfactory receptors were shotgun cloned and sequenced. From these BAC sequences, two ora genes and 55 OlfC genes were identified in Atlantic salmon. The second technique used to identify olfactory receptors in Atlantic salmon was a bioinformatic approach that involved screening a 3-fold Atlantic salmon genome sequence for olfactory receptors. Using this approach, 24 MOR and the remaining five ora genes were identified, as well as another 24 partial genes or pseudogenes. As a first step to understand how olfactory receptors are involved in imprinting and homing, a suite of olfactory receptors were selected to examine the expression profiles of these genes across different life stages and life histories of wild Atlantic salmon from Newfoundland, Canada. Seven differentially expressed OlfC genes were identified in juvenile anadromous salmon compared to returning adult salmon. From this research, I hypothesize that OlfC genes may play an important role in the imprinting of home stream water olfactory cues in anadromous Atlantic salmon.

Document type: 
Thesis
File(s): 
Supervisor(s): 
William Davidson
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) Ph.D.

Generation and Analysis of Suppressors of Caenorhabditis Elegans Unc-2 Alleles

Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2004
Document type: 
Thesis
File(s): 
Supervisor(s): 
David L. Baillie
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) M.Sc.

HSP90 and NO/cGMP in the development of ascidians and echinoids

Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2002
Document type: 
Thesis
File(s): 
Supervisor(s): 
Bruce P. Brandhorst
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Dissertation) Ph.D.

Molecular basis of Bardet-Biedl syndrome with particular reference to the Newfoundland families

Author: 
Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2004
Document type: 
Thesis
File(s): 
Supervisor(s): 
William S. Davidson
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Dissertation) Ph.D.

Computational detection of transcriptional regulators of protein complexes in apoptosis

Author: 
Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2004
Document type: 
Thesis
File(s): 
Supervisor(s): 
Frederic Pio
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) M.Sc.

The fate of hapthenic acids in wetland sediments : microbial metabolism and community structure

Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2004
Document type: 
Thesis
File(s): 
Supervisor(s): 
Margo Moore
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) M.Sc.

Peptide markers for the HIV-1 neutralizing antibody 4E10

Author: 
Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2004
Document type: 
Thesis
File(s): 
Supervisor(s): 
Jamie K. Scott
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) M.Sc.

Genetic characterization of Nemo shows complex genetic interactions with the Egfr pathway

Author: 
Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2004
Document type: 
Thesis
File(s): 
Supervisor(s): 
E. Verheyen
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) M.Sc.

Requirement for Wnt and Planar Cell Polarity pathway genes in asymmetric neuroblast division in Caenorhabditis elegans

Author: 
Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2004
Document type: 
Thesis
File(s): 
Supervisor(s): 
Nancy C. Hawkins
Department: 
Science: Department of Molecular Biology and Biochemistry
Thesis type: 
(Thesis) M.Sc.

The drosophila gene Nemo regulates multiple developmental pathways

Author: 
Peer reviewed: 
No, item is not peer reviewed.
Date created: 
2004
Document type: 
Thesis
File(s): 
Supervisor(s): 
Esther Verheyen
Department: 
Science: Molecular Biology and Biochemistry Department
Thesis type: 
(Thesis) M.Sc.